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Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression <t>of</t> <t>Nfatc1,</t> C-fos, Atp6v0d2, <t>Ctsk,</t> Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).
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Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression <t>of</t> <t>Nfatc1,</t> C-fos, Atp6v0d2, <t>Ctsk,</t> Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).
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Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression <t>of</t> <t>Nfatc1,</t> C-fos, Atp6v0d2, <t>Ctsk,</t> Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).
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Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression of Nfatc1, C-fos, Atp6v0d2, Ctsk, Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).

Journal: Materials Today Bio

Article Title: An alkaline nanosized platelet vesicle-based hydrogel for the treatment of osteoporotic bone defects

doi: 10.1016/j.mtbio.2026.103000

Figure Lengend Snippet: Inhibitory effects of NaHCO 3 @NPVs on osteoclast differentiation and function in vitro . (A, B) TRAP staining and statistical analysis of the number and area percentage of multinucleated TRAP-positive osteoclasts (scale bar, 200 μm; n = 4 per group). (C) Real-time quantitative polymerase chain reaction (RT-qPCR) results showing that NaHCO 3 @NPVs significantly downregulate the expression of Nfatc1, C-fos, Atp6v0d2, Ctsk, Acp-5, and Mmp9 (n = 3 per group). (D, E) Scanning electron microscopy images and statistical analysis of bone resorption area (scale bars, 200 μm and 50 μm; n = 6 per group). (F, G) Western blot analysis confirming that NaHCO 3 @NPVs inhibit osteoclast differentiation and function (NFATc1, c-Fos, and CTSK) (n = 3 per group). (H) F-actin staining of mature osteoclasts (scale bar, 100 μm).

Article Snippet: Western blotting was used to evaluate the protein expression of NFATc1 (sc-7294, Santa Cruz), c-Fos (sc-166940, Santa Cruz), CTSK (11239-1-AP, ProteinTech), and β-actin (20536-1-AP, ProteinTech).

Techniques: In Vitro, Staining, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Electron Microscopy, Western Blot